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Female candidates get 1 extra year for each child born since receiving the PhD. The ECMTB Organizing Committee is setting up a Mentorship Program to facilitate research and career interactions between junior and senior scientists attending the meeting. Participants of ECMTB can sign up to be part of the mentorship program, either as a mentee, a mentor, or both. EMS is a conference where statisticians of all ages and from all regions meet to exchange ideas and talk about the newest developments on the broad field of statistics and probability theory.

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For Bernoulli Society sessions specifically, an effort will be made to strike a proper balance between statistics and probability in light of the typical composition of the body of WSC attendees. The post is funded for 36 months, starting 1 July, or by agreement. Opening: Martin Wainwright. Forum: Mark Girolami.

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Closing: Yann LeCun. The idea of the meeting is to provide young researchers an introduction to the international scene within the broad subject area - from pure probability theory to applied statistics. Participation is by invitation only. It will address theoretical, methodological and practical aspects of this field. Bhattacharya, Lawrence D.

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This course presents an introduction and recent advances in high dimensional statistics, with a special emphasis on main concepts of variable selection, nonparametric estimation, supervised and unsupervised classification and multiple testing. This conference will bring together probabilists studying the evolution of complex random systems such as spin glasses, random matrices, growth process, and stochastic PDEs.

It will also include a celebration of the 60th birthday of Gerard Ben Arous. The program includes a graduate summer school, a research program, an undergraduate summer school , an undergraduate faculty program, a teacher leadership program, and a workshop on increasing minority participation in undergraduate mathematics.

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Undergraduate courses given by Antonio Auffinger, and Mihai Stoiciu. The aim of ICORS is to bring together researchers and practitioners interested in robust statistics, data analysis and related areas.

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This summer school will be held at the Institut des Hautes Etudes Scientifiques, and is open to everybody but intended primarily for young participants, including PhD students and postdoctoral fellows. Job offers can range from graduate scholarships to full Professor positions, in Probability, Mathematical Statistics, and related areas. Those who are aware of open calls may send a contribution directly to the e-Briefs Editor, including an URL where the complete information is available. The Department of Probability and Statistics at CIMAT is welcoming applications towards a tenure-track position in the area of Statistics, broadly understood, beginning between July, to July, Preference will be given to candidates with excellent accomplishments in research in these areas or with outstanding potential, with no more than 15 years from graduation.

Deadline: April This is a fixed-term position, focusing manly on teaching across a range of statistics courses, but with opportunities to take part in research activities. The post is fixed term until 31 of August Deadline for applications to be sent to This email address is being protected from spambots. Bernoulli Society Bulletin e-Briefs issue February It was a pleasure to serve as the e-Briefs editor in the past two years. Let me welcome Corina Constantinescu who has co-edited this issue with me and who will take over from issue Leonardo T.

Registration to open soon. Deadline for contributed talk and poster proposal: February The SSP is a series of annual conferences devoted to stochastic analysis, Markov processes and other topics in probability theory of current interest. Tutorial lectures: Elchanan Mossel. Deadline for abstract submission: February Deadline for abstract submission: March Deadline for applying: March The University of Edinburgh will make a substantial number of appointments in the Mathematical Sciences as part of an ambitious plan for the School of Mathematics. As part of this development, we wish to appoint two Lecturers in Statistics and Data Science to start on 1 August or by agreement.

We are seeking candidates with a track record of high quality research. The successful applicants will contribute to the establishment of the University of Edinburgh as an international centre for Statistics and Data Science, and will deliver high quality undergraduate and postgraduate level teaching. S7 Table. Similarly, 11 genes were negatively regulated by XibR but activated by low-iron condition Fig 3B. S8 Table. These included 13 iron uptake and metabolism related genes, 4 pathogenicity associated genes, 14 secretion components and effectors, 14 flagellar and motility related genes, 4 attachment associated genes, 10 chemotaxis related genes and 18 two component systems associated genes, respectively Fig 3B ; S3 Table.

Similarly, we also identified genes which were repressed by XibR but not influenced by low-iron condition Fig 3B ; S4 Table. Analysis of our microarray data indicated that several genes related to iron storage ferritin-like proteins , putative outer membrane receptor for ferric iron uptake fhuE and putative TonB dependent receptors were positively regulated by XibR S3 Table ; S9 Table.

Transport was initiated by addition of 0. Transport was initiated by the addition of 0. E Streptonigrin SNG sensitivity plate assay.

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F Intracellular iron content quantification determined by atomic absorption spectrophotometry. Different Xcc strains were grown at a density of 1.

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We next performed streptonigrin sensitivity assay, which depends on the intracellular iron levels, to assess intracellular iron content in different strains of Xcc [ 13 , 32 ]. In order to examine this, we made three deletion strains; 1. S9I Fig. It has been reported that Xanthomonas oryzae pv. Interestingly, the wild-type Xcc strain grown under low-iron condition exhibited significantly higher RCR in response to xylose and glutamic acid, compared to those grown in PS medium.

Next we wanted to examine whether low-iron condition promotes motility in Xcc. E Motility zone diameter quantification from semisolid swim plate motility assay.

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G Bacterial velocity measurement from the live cell imaging of bacterial movement at single cell level by using manual tracking and chemotaxis tools with ImageJ software. The stained cells were loaded into a chamber of sterile glass bottom plates containing PS medium with 0. Values are mean of at least 25 bacteria up to 20 frames. The experiment was repeated three times. Error bars are SEM. A Schematic representation of ChIP-qPCR primer locations indicated by arrows relative to the transcriptional start sites of mot , flg , and xss operons. Specific binding is indicated by a loss of XibR binding to the radiolabelled probe in the presence of excess of cold probe indicated by CP.

In the presence of a nonspecific probe negative control , XibR did not exhibit binding. Y-axis indicates molar ellipticity. Further, we performed quantification of bacterial cells attached to polystyrene culture plates after 24 hours of inoculation by crystal violet CV staining, as described previously [ 34 ]. We identified a homolog of previously reported E. However, there was no significant difference under low-iron condition compared to the PS medium Fig 6E. B Quantification of attached cells of different Xcc strains in the static biofilm after 24 hours of growth.

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Attached cells were stained with Crystal Violet CV , dissolved in ethanol and quantified by measuring absorbance at nm. Each 3D image represents the layer in the Z-stack.

D Average biofilm thickness of different strains of Xcc formed on the glass slide at the air-media interphase. Our expression analysis indicated that XibR is involved in the regulation of mot , flg , and xss operons. DNA-protein cross-linking was done by formaldehyde treatment to bacterial cells followed by immunoprecipitation of XibR from lysates using anti-HA antibodies by a sandwich technique see Materials and Methods. Interestingly, XibR exhibited significant binding to xss promoter only under iron-replete condition Fig 7D. Since under aerobic conditions, ferrous iron is oxidized to ferric form of iron, and to rule out the possibility that the increased binding of XibR exhibited in the presence of ferrous iron may be due to conversion to ferric form, we added ferric specific chelator deferoxamine mesylate in the binding buffer.

Sequence analysis of bp upstream region of all the XibR regulated genes identified in the microarray experiment indicated that Furthermore, we performed circular dichroism CD spectroscopy to detect possible structural changes in XibR in the presence of ferric iron. For infection studies, day old cabbage leaves were inoculated with bacterial cell suspension by leaf clip method and monitored the lesion development, bacterial growth and migration inside the leaves Fig 8.

A Infected cabbage leaves with different Xcc strain showing symptoms as a lesion at 15 days postinoculation. Bacterial populations were measured by crushing the leaves of 1cm 2 areas for each and serial dilution plating at the indicated post inoculation days. C In planta bacterial migration assay was performed by inoculating 1cm pieces of infected leave, cut from base to tip with sterile scissors on rich PS medium with respective antibiotics.

Migration was estimated by observing colonies formed after 1 to 3 days by the bacterial ooze from the cut ends of cabbage leaf pieces. D Quantification of lesion length at 15 days post inoculation. Ability of the bacterial pathogen to respond and adapt to iron limiting condition inside host is essential to their virulence.

Pathogenic bacteria utilize diverse and efficient iron uptake systems that enable them to scavenge various forms of iron from the environment under iron-restricted conditions. Due to essentiality of iron in bacterial growth and survival particularly in iron limiting environment and also the potential toxic effect due to iron overload, bacteria tightly regulate iron uptake, metabolism and distribution in response to environmental cues mediated by iron dependent regulators such as Fur and DtxR [ 4 , 7 , 9 ].

Fur and Fur-like homologs play an important role in regulating iron metabolism and virulence in many Gram-negative bacteria. Fur also has been shown to act as an activator of virulence associated factors in bacterial pathogens such as Neisseria meningitidis , Salmonella typhimurium and Helicobacter pylori [ 9 ]. However, apart from Fur and DtxR type ferrous binding regulators, little is known about how iron regulated genes and virulence associated functions are fine-tuned and coordinately regulated by other iron responsive regulatory proteins.

In this study we showed that a novel NtrC family of response regulator, XibR binds to ferric form of iron and regulates the expression of several iron metabolism and virulence associated functions in important phytopathogen Xanthomonas campestris pv. To our knowledge iron-responsive ferric binding regulator has not been reported in any bacteria.